A federal judge on Monday blocked the Biden administration’s new Title IX regulations in six more states as Republicans and conservative groups try to overturn a policy that expanded protections for L.G.B.T.Q. students.

In a 93-page opinion, Judge Danny C. Reeves of the Eastern District of Kentucky ruled that the Education Department had overreached in expanding the definition of “sex” to include gender identity.

Judge Reeves halted the regulations in Tennessee, Kentucky, Ohio, Indiana,…